Effects of hemoglobin level on Rho kinase of endothelial cells and tight junction protein claudin-5 in blood-brain barrier of rats after intracerebral hemorrhage
10.3760/cma.j.issn.1671-8925.2013.06.004
- VernacularTitle:脑出血后血红蛋白对血脑屏障内皮细胞Rho激酶Ⅱ及紧密连接蛋白claudin-5的影响
- Author:
Xin-Qing DENG
1
;
Yi-Zhao CHEN
;
Shuo YANG
;
Bing LI
;
Wei-Ping JIANG
;
Zheng-Hao FU
;
Xiao-Dan JIANG
Author Information
1. 510280广州,南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点实验室
- Keywords:
Iintracerebral hemorrhage;
Hemoglobin;
Blood-brain barrier;
Rho kinase Ⅱ;
Tight junction claudin-5
- From:
Chinese Journal of Neuromedicine
2013;12(6):555-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hemoglobin level on Rho kinase Ⅱ of endothelial cells and tight junction protein clandin-5 in blood-brain barrier (BBB) of rats after intracerebral hemorrhage (ICH).Methods Male SD rats were randomized into normal control group (n=18) and hemoglobin inducement group (n=72); hemoglobin inducement group was divided into 4 subgroups (24 and 48 h,and 3 and 7 d after surgery,n=18).Rat ICH models in the hemoglobin inducement group were established by hemoglobin injection into the brain tissues.The expression ofRho kinase Ⅱ of endothelial cells and phosphorylated myosin light chain protein in BBB were observed by immunohistochemical staining,and BBB permeability was evaluated by Evans Blue dye; The protein and mRNA expressions of claudin-5 in the perihematomal brain tissues were detected by immunofluorescence staining and real-time quantitative PCR.Results Immunohistochemical staining indicated that expressions of Rho kinase Ⅱ of endothelial cells and phosphorylated myosin light chain in BBB of normal control group were rare,and those in the hemoglobin inducement group were high; as compared with that in the normal control group,mean optical density of Rho kinase Ⅱ and phosphorylated myosin light chain proteins in the endothelial cells of BBB in hemoglobin inducement group increased significantly at 48 h and 3 d after inducement (P＜0.05).The permeation of Evans Blue in hemoglobin inducement group significantly increased (P＜0.05).Immunofluorescence staining showed that the expression of clandin-5 in hemoglobin inducement group was uncontinuous and low as compared with that in the normal control group at 24 and 48 h and 3 d after inducement.PCR showed that the mRNA expression of claudin-5 in hemoglobin inducement group was significantly lower than that in the normal control group at 24 and 48 h and 3 and 7 d after the inducement (P＜0.05).Conclusion After intracerebral hemorrhage,hemoglobin can lead to increased expression of phosphorylated myosin light chain,and reduce the expression of tight junction protein claudin-5 of BBB by increasing the expression ofRho kinase Ⅱ in endothelial cells of BBB,which may be an important mechanism in the disruption of BBB and the occurrence of brain edema after intracerebral hemorrhage.
