Effect of hemoglobin on blood-brain barrier in rat models after intracerebral hemorrhage
10.3760/cma.j.issn.1671-8925.2013.04.009
- VernacularTitle:脑出血后血红蛋白对血脑屏障的影响
- Author:
Shuo YANG
1
;
Yi-Zhao CHEN
;
Xin-Qing DENG
;
Wei-Ping JIANG
;
Bing LI
;
Zheng-Hao FU
;
Yi-Quan KE
Author Information
1. 510282广州,南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点实验室
- Keywords:
Hemoglobin;
Blood-brain barrier;
Tight junction;
Brain edema
- From:
Chinese Journal of Neuromedicine
2013;12(4):364-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hemoglobin on structure and function of blood-brain barrier (BBB) in rat models after intracerebral hemorrhage.Methods One hundred and eight male SD rats were randomized into normal control group (n=12),intracerebral hemoglobin injection group (Hb group,n=48) and sham-operated group (n=48); according to the different observation time points,rats in the Hb group and sham-operated group were divided into 4 subgroups (6 and 24 h,3 and 7 d after the injection,n=1 2).Histological changes and iron deposition of the brain tissues were examined with HE staining and iron staining,respectively.BBB permeability was evaluated by the permeation of Evens blue.The expressions of claudin-5 and ZO-1 in perihematomal brain tissues were detected by immunofluorescence staining and real-time fluorescence quantitative PCR.Results Evident edema and necrosis were observed in the perihematomal zone of Hb group,and iron deposition was found 3 and 7 days after the injection.The permeation of Evens blue in Hb group was significantly increased as compared with that in sham-operated group (P＜0.05).Immunofluorescence staining indicated that expressions ofclaudin-5 and ZO-1 were low and discontinuous in Hb group; the mRNA expression levels of claudin-5 and ZO-1 in Hb group were significantly lower than those in the sham-operated group (P＜0.05).Conclusion After intracerebral hemorrhage,Hb may induce the damage of BBB and participate in the course of brain edema.
