Role of insular cortex in amygdala-kindled seizures in rats
10.3760/cma.j.issn.1671-8925.2013.02.003
- VernacularTitle:岛叶在杏仁核点燃大鼠中的作用
- Author:
Xue-Feng ZHANG
1
,
2
;
Feng WANG
;
Lu MIAO
;
Zi-Mu SONG
;
En-Jie WANG
;
Jin XU
;
Tao SUN
Author Information
1. 750004银川,宁夏医科大学颅脑疾病重点实验室
2. 264003烟台,滨州医学院烟台附属医院
- Keywords:
Insular cortex;
Amygdala;
Hippocampus;
Kindling;
Activity-regulated cytoskeleton-associated protein
- From:
Chinese Journal of Neuromedicine
2013;12(2):119-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of insular cortex in amygdala-kindled seizures in rats.Methods Forty-eight healthy male SD rats were randomly divided into blank control group (n=8),sham-operated group (n=8) and amygdala-kindled group (n=32); no treatment was performed in the blank control group,and only implantation of electrodes was performed in the sham-operated group; implantation of electrodes and electrophotoluminescence were performed in the amygdala-kindled group to induce amygdala-kindled seizure models.Rats in the amygdala-kindled group was divided into 4sub-groups (n=8) at different times after the kindling (1,3,6 and 12 h).Fluorescence in situ hybridization and immunohistochemistry staining were employed to investigate the altered mRNA and protein expressions of activity-regulated cytoskeleton-associated protein (Arc) in the hippocampus and insula of the rat brain.Results As compared with that in the blank control group and sham-operated group,Arc mRNA expression in the amygdala-kindled sub-groups increased at 1 h after the kindling (P＜0.05),peaked at 3 h after the kindling (P＜0.05),and returned to basic level at 6 h after the kindling (P＞0.05).As compared with that in the blank control group and sham-operated group,Arc protein expression in the amygdala-kindled sub-groups increased at 3 h after the kindling,(P＜0.05),peaked at 6 h after the kindling (P＜0.05),and returned to basic level at 12 h after the kindling (P＞0.05).No significant difference on mRNA and protein expressions of Arc was noted between the sham-operated group and blank control group (P＞0.05).Conclusion Insular cortex,the amygdala and the hippocampus form a focus complex,which participates in the occurrence of temporal lobe epilepsy.
