Role of NADPH oxidase in brain damage oxidative stress in ATP7Btx-J mice
10.3760/cma.j.issn.1671-8925.2012.11.010
- VernacularTitle:NADPH氧化酶在ATP7Btx-J小鼠脑损伤氧化应激过程中的作用
- Author:
Qian WEI
1
;
Xiao-Qing LIU
;
Xian-Ting JIAO
;
Yong-Jun ZHANG
;
Xiao-Gang YU
Author Information
1. 上海交通大学医学院附属新华医院儿内科
- Keywords:
Wilson Disease;
Superoxide anion;
NADPH oxidase;
Cleaved caspase-3;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2012;11(11):1126-1130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether NADPH oxidase is involved in brain damage of ATP7Btx-J mice through interfering oxidative stress.Methods ATP7Btx-J mice (20 weeks old),wild-type (WT) mice (20 weeks old) and apo-ATP7Btx-J mice (given the NADPH oxidase inhibitor apocynin) were chosen in our study; apo-ATP7Btx-J mice were treated by daily oral administration with 200 mg/kg of apocynin since 16 weeks old till 20 weeks old.Copper concentration was determined by inductively coupled plasma-mass spectrometry (ICP-MS) and NADPH oxidase activity was detected by colorimetric method.The superoxide level was measured using superoxide-sensitive fluorescent probe dihyroethidine (DHE).The protein expression level of Cleaved caspase-3 was analyzed by Western blotting.The level of neuronal apoptosis was assayed with TUNEL method.Results The copper content in the striatum region of ATP7Btx-J mice was significantly higher than that of wild-type (WT) mice (P＜0.05).The activity ofNADPH oxidase and concentration of superoxide anion in the striatum region of ATP7Btx-J mice were significantly higher than those of WT mice (P＜0.05); those in the striatum region of apo-ATP7Btx-J mice were significantly lower than those of ATP7Btx-J mice (P＜0.05).The protein expression of Cleaved caspase-3 and the level of neuronal apoptosis in the striatum region of ATP7Btx-J mice were significantly higher than those of WT mice (P＜0.05); those in the striatum region of apoATP7Btx-J mice were significantly lower than that of ATP7Btx-J mice (P＜0.05).Conclusion NADPH oxidase may play a role in neuronal apoptosis in the striatum region of ATP7Btx-J mice through oxidative stress,and apocynin can protect the nervous system decreasing the NADPH oxidase level.
