Influence of pomegranate polyphenols in cognitive impairment of chronic cerebral hypo-perfused rats
10.3760/cma.j.issn.1671-8925.2012.11.003
- VernacularTitle:石榴多酚对慢性脑低灌注大鼠认知功能损害的影响
- Author:
Li LIU
1
;
Jin-Song XIAO
;
Xing-Gui LI
;
Ying YANG
;
Zi-An ZHONG
;
Hui LIU
Author Information
1. 武汉大学中南医院神经内科
- Keywords:
Pomegranate polyphenol;
Chronic cerebral hypo-perfusion;
Oxidative stress;
Cognitive impairment
- From:
Chinese Journal of Neuromedicine
2012;11(11):1088-1092
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the influence of pomegranate polyphenols in cognitive impairment of chronic cerebral hypo-perfused rats.Methods Healthy male Wistar rats were selected in our study and randomly divided into sham-operated group,bilateral common carotid artery occlusion/two-vessel occlusion group (2VO control group) and 2VO+PJ (pomegranate Juice) group.The chronic cerebral hypo-perfused rat models were established by permanent occlusion of bilateral common carotid arteries of rats; one week after surgery,the rats in the first two groups were fed with sugar water and those in the last group were fed with pomegranate juice at a dosage of 2 mL/d by gavage for 7 weeks.Then,spatial learning and memory abilities were assessed using Morris water maze test.After that,the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the cortex and hippocampus were assessed by biochemical determinations.The protein expression level of 4-hydroxynonenal (4-HNE) was detected by Western blotting.Results The time of escape latencies in 2VO control group was longer while the time spent in object square was shorter than those of sham-operated group (P＜0.05); the time spent in the object square of 2VO+PJ group ([6.18±1.32] s) was significantly longer as compared with that of 2VO control group ([3.96±1.65] s,P＜0.05),and that of the escape latencies was shorter as compared with that of 2VO control group (P＜0.05).The SOD levels in the 2VO+PJ group (the cortex:[19.79±3.34] U/mg prot,the hippocampus:[20.87±0.77] U/mg prot) were significantly higher than those of 2VO control group (the cortex [15.79±0.39],the hippocampus [18.90±0.81],P＜0.05).The MDA levels in the 2VO+PJ group (the cortex:[1.21±0.29] nmol/mg prot,the hippocampus [2.31±0.35] nmol/mg prot)were obviously lower than those of 2VO control group (the cortex [1.74±0.42],the hippocampus [2.86±0.51],P＜0.05).The 4-HNE level in the 2VO+PJ was significantly lower than that of 2VO control group (P＜0.05).Conclusion Pomegranate polyphenol can improve the cognitive impairment in rats after chronic cerebral hypoperfusion,which may be related to its anti-oxidative effect.
