Intraarterial injection of bone marrow mesenchymal stem cells: a safety study
10.3760/cma.j.issn.1671-8925.2012.10.008
- VernacularTitle:经颈内动脉移植骨髓间充质干细胞的安全性研究
- Author:
Fei YANG
1
;
Jian-Wei JIANG
;
Peng WANG
;
Ling WANG
;
Xiao-Xing FENG
;
Jun-Gan WANG
;
Qiu-Ju HUANG
;
Hai-Jing YU
Author Information
1. 南通大学第三附属医院介入放射科
- Keywords:
Bone mesenchymal stem cell;
Cell transplantation;
Internal cartid artery;
X-ray computed tomography;
Cerebral infarction
- From:
Chinese Journal of Neuromedicine
2012;11(10):1002-1005
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether mesenchymal stem cells derived from bone marrow (BMSCs) could be administered safely into the internal carotid artery.Methods Density gradient centrifugation was employed to extract the BMSCs.About 1 × 106 BMSCs were injected into the internal carotid artery of healthy New Zealand rabbits through cerebral angiography.After 24 h of injection,we checked the cerebral microcirculation with CT perfusion and possibly microinfarction with HE stainning.Results Successful administration of BMSCs into internal carotid artery of the rabbits was performed.No significant difference was found in perfusion parameters,including cerebral blood flow (CBF),cerebral blood volume (CBV),mean transit time (MTT),and time to peak (TTP),between transplanted hemisphere and contralateral hemisphere (P＞0.05),despite a trend of slight decrease of CBF in the transplanted side.HE staining showed no microinfarctions in cortex-medulla junctional zone of the transplanted hemispheres.Conclusion BMSCs could be transplanted safely through internal carotid artery of the rabbits.
