Expression of interleukin -32 in glioma and its regulatory mechenism
10.3760/cma.j.issn.1671-8925.2012.09.002
- VernacularTitle:IL-32在神经胶质瘤中的表达及调节机制研究
- Author:
Zhi-Yuan MA
1
;
You-Feng GUO
;
Yan CHEN
;
Chun-Hong GUAN
;
Shu-Ming HUANG
Author Information
1. 广东省湛江市第二人民医院
- Keywords:
Glioma;
Interleukin 32;
Expression
- From:
Chinese Journal of Neuromedicine
2012;11(9):869-872
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the expression of interleukin-32 (IL-32) in glioma and its regulatory mechanism. Methods RT-PCR and Western blotting were used to detect the mRNA and protein expression levels of IL-32 in CHG-5 and U251 cell lines in vitro cultured for 3 d.And then, 10 ng/mL IL-1β, IL-4, IL-6, IL-10, IL-17, tumour necrosis factor alpha (TNF-α), TNF-β and interferon-gamma(IFN-γ) were put into U251 cell lines for 24 h,and RT-PCR was employed to detect the mRNA expression of IL-32; meanwhile,RT-PCR was also employed to detect the mRNA expression of IL-32 after the treatment of IL- 1 β,TNF-α and IFN-γ for different times,respectively. Results The mRNA expression of IL-32 in U251 cells was (6.41±1.12)-fold higher than that in CHG-5 cells (P＜0.05);the protein expression level of IL-32 in U251 cells (0.95±0.42) was significantly higher than that in CHG-5 (0.28±0.13,P＜0.05).IL-1β,TNF-α and IFN-γ markedly enhanced the IL-32 mRNA expression at 24 h after treatment as compared with that before treatment, and dose- and time-dependent manners of IL-32 mRNA expression were noted (P＜0.05). Conclusion High expression of IL-32 is noted in glioma; IL-1β, TNF-α and IFN-γ can regulate the IL-32 mRNA expression with dose- and time-dependent manners.
