Immunohistochemical results of cavernous malformation of the central nervous system
10.3760/cma.j.issn.1671-8925.2012.08.011
- VernacularTitle:中枢神经系统海绵状血管畸形的免疫组化染色研究
- Author:
Chu-Wei CAI
1
;
Li-Jin HUANG
;
Jun WEN
Author Information
1. 汕头市中心医院
- Keywords:
Cavernous malformation;
Immunohistochemistry;
Central nervous system
- From:
Chinese Journal of Neuromedicine
2012;11(8):799-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the endothelial cell markers,angiogenesis and proliferative activity ofcavemous malformation (CM) ofthe central nervous system by immunohistochemical study.Methods Ninety-seven patients with CM performed excision and confirmed by pathologic examination from April 2004 to August 2008 were chosen in our study.According to the MRI manifestations and their pathological features,they were divided into bleeding group (n=47) and non-bleeding group (n=50); and 20 normal brain samples from patients of open cranial-cerebral trauma were chosen as controls. All samples were stained by immunohistochemistry with CD34, α-smooth muscle actin (α-SMA), and vascular endothelial growth factor (VEGF),respectively. Results CD34 expressed in the endothelium of CM discontinuously; in CM with small lumen,thick layer of immunohistochemistry with CD34 was noted,while in CM with large lumen,thin layer of that was observed discontinuously; and the α-SMA expressed in the sub endothelium of CM with diffuse distribution. VEGF expressed obviously in endothelium,sub endothelium and intervascular spaces with diffuse distribution; significant differences of them were noted between each 2 groups (control group,bleeding group and non-bleeding group,P＜0.05).Ki-67 was negative in all samples. Conclusion Lack of vessel smooth muscle may relevant to bleeding tendency of CM; the up-regulation of VEGF may be the result of bleeding stimulation and absence of Ki67 indicates no proliferation in CM.
