Proteomic profiling and identification of malignant grade related proteins in human brain astrocytoma
10.3760/cma.j.issn.1671-8925.2012.08.006
- VernacularTitle:人脑星形胶质细胞瘤恶性程度相关蛋白表达谱的分析与鉴定
- Author:
Jin-Qiao ZHOU
1
;
Jing-Tao WANG
;
Qiu-Hong LIU
;
Xin-Bin GUO
;
Jian ZHOU
;
Lai-Jun SONG
Author Information
1. 郑州大学附属第一医院
- Keywords:
Astrocytoma;
Grade malignancy;
proteomics;
Two-dimensional electrophoresis;
Tumor biomarker
- From:
Chinese Journal of Neuromedicine
2012;11(8):780-783
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the malignant grade related proteins implicated in human astrocytoma with proteomic techniques. Methods Two-dimensional electrophoresis (2-DE) was used to compare the protein expression profiles of normal human brain tissues (n=12) and astrocytoma tissue samples of various malignant grades (23 with grade Ⅱ, 15 with grade Ⅲ and 14 with grade Ⅳ ).After identifying the differential protein spots, electrospray ionization (ESI) tandem mass spectrum and bioinformatics were employed to determine the protein ID. Results A total of 15 proteins with differential expressions were identified,which included 10 up-regulated ones (heat shock protein 27,prohibitin,78-kDa glucose-regulated protein,peroxiredoxin 1,peroxiredoxin 6,annexin Ⅱ,cathepsin D,plasminogen activator inhibitor-1, actin [cytoplasmic 1], Glutathione S-transferase M) and 5 down-regulated proteins (disulfide isomerase A3 protein,alpha-crystallin B chain protein,T-complex protein 1 ε subunit,ubiquitin carboxyl-terminal hydrolase isozyme L1 and glial fibrillary acidic protein) in astrocytoma tissues of grade Ⅲ and Ⅳ. Conclusion Proteomic techniques can screen the malignant-grade associated proteins effectively,which provides rationale for individualized diagnosis and therapeutic targets of astrocytoma.
