Proteomic analysis of autologous bone marrow derived neural stem cell transplantation for treatment of central nervous system injury
10.3760/cma.j.issn.1671-8925.2012.07.011
- VernacularTitle:骨髓源神经干细胞移植治疗中枢神经系统损伤的蛋白质组学研究
- Author:
Can MA
1
;
Yi-Wu DAI
;
Ru-Xiang XU
Author Information
1. 安徽医科大学北京军区总医院
- Keywords:
Central nervous system injury;
Proteomics;
Stem cell transplantation;
Cerebrospinal fluid
- From:
Chinese Journal of Neuromedicine
2012;(7):691-694
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To apply the isobaric tagging for relative and absolute quantitation (iTRAQ) and liquid chromatography-tandem mass spectromeury (LC-MS/MS) to identify the proteins differentially regulated in CSF of patients before and after stem cell transplantation.[Methods]CSF samples fiom patients with central nervous system injury before and after stem cell transplantation and from normal controls were collected;selective removal of high-abundant proteins was performed by high performance liquid chromatography;proteins were differentially identified by iTRAQ-coupled LC-MS/MS.[Results] Totally,59 proteins were identified.As compared with control group,the patients before transplantation had 5 over-expressed proteins and 8 under-expressed proteins;only 7 proteins in patients after transplantation showed no significantly different expression as compared with those in control group.[Conclusion] Protein expression is significantly changed in CSF of patients with central nervous system injury after neural stem cell transplantation,suggesting that the proteomics research can help us to find markers of nervous system damage and is important to mechanism study on nervous system damage and repair.
