Adenovirus-mediated glucose transporter 3 infection in PC12 cells of rats after hypoxiareperfusion injury
10.3760/cma.j.issn.1671-8925.2012.07.002
- VernacularTitle:Ad-GLUT3转染对缺氧再灌注损伤PC12细胞的影响
- Author:
Zhong-Hua WU
1
;
Xi-Wen SHI
;
Jun-Liang LI
;
Bin WANG
;
Yong-Ming LI
;
Fang-Cheng LI
Author Information
1. 河南省人民医院
- Keywords:
Hypoxia and reperfusion injury;
Glucose transporter 3;
Glucose uptake rate;
Apoptosis;
Nuclear factor-kB
- From:
Chinese Journal of Neuromedicine
2012;(7):653-657
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the effect of adenovirus-mediated glucose transporter 3 (Ad-GLUT3) infection on PC 12 cells of rats after hypoxia-reperfusion injury.[Methods]PC 12 cells treated with hypoxia and re--oxygenation were prepared;after PC12 cells being infected by Ad-GLUT3 (experimental group),adenovirus encoding green fluorescent protein (Ad-GFP group) and blank plasmid (control group),respectively,flow cytometry (FC) and TUNEL assay were used to analyze the apoptosis of PC12 cells;the glucose uptake rate was detected by isotopic-scintiscan,and the expression of nuclear factor (NF-kB) was detected by Western blotting.[Results] FC indicated that the apoptotic rate in the experimental group (4.7％±1.75％) was significantly lower as compared with that in the Ad-GFP group (14.25％±3.46％) and control group (13.1％±2.98％,P＜0.05);TUNEL assay showed that the apoptotic rate in the experimental group (7.7％±3.31％) was significantly lower as compared with that in the Ad-GFP group (17.1％±3.10％) and control group (15.2％±2.70％,P＜0.05).And the glucose uptake rate in the experimental group (603％±13.1％) was obviously higher as compared with that in the Ad-GFP group (300％±8.77％) and control group (319％±11.2％,P＜0.05);and the NF-k＜B level in the experimental group (0.92±0.17) was significantly lower as compared with that in the Ad-GFP group (1.02±0.24) and control group (1.06±0.21,P＜0.05).[Conclusion] After being infected by Ad-GLUT3,the PC12 cells treated with hypoxia and re-oxygenation enjoy elevated glucose uptake rate and inhibited apoptotic rate.
