Distribution and expression changes of tight junctional protein JAM-1 in rat models after intracerebral hemorrhage
10.3760/cma.j.issn.1671-8925.2012.07.001
- VernacularTitle:实验性脑出血后紧密连接蛋白JAM-1的分布与表达变化
- Author:
Wei-Ping JIANG
1
;
Yi-Zhao CHEN
;
Bing LI
;
Shuo YANG
;
Xin-Qing DENG
;
Zheng-Hao FU
;
Mou-Xuan DU
;
Yan-Ping TANG
;
Yi-Quan KE
Author Information
1. 南方医科大学珠江医院
- Keywords:
JAM-1;
Intracerebralhemorrhage;
Tight junction;
Blood-brain barrier;
Inflammation
- From:
Chinese Journal of Neuromedicine
2012;(7):649-652
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the distribution and expression changes of tight junctional protein JAM-1 in rat models after intracerebral hemorrhage (ICH) and their significance.[Methods]One hundred and twenty-eighty healthy male SD rats were randomly divided into normal control group (n=16) and ICH group (n=112),and the ICH models were induced by stereotactically injecting 75 uL autologous blood into the right caudate nucleus.Seven time points after ICH (6,12,24 and 48 h,and 3,7 and 14 d after ICH,16 rats for each time point) were chosen.BBB permeability was evaluated by Evans blue dye extravasation.The distribution and expression of JAM-1 were detected by immunofluorescence and real-time quantitative PCR.[Results] As compared with that in the normal control group,BBB permeability in the ICH group significantly increased at 24 and 48 h,and 3 and 7 d after ICH (P＜0.05).JAM-1 expression decreased at blood vessels at 12,24 and 48 h after ICH,and JAM-1 expressed at the circulatingleukocytes3 dafterlCH,and abundant JAM-1 positive cells around hematoma were noted in the ED-l-positve macrophages 7 d after ICH.JAM-I mRNA significantly decreased at 12,24 and 48 h after ICH,and significantly increased 7 d after ICH as compared with that in the normal control group (P＜0.05).[Conclusion] JAM-1 experssion changes not only participate in regulation of BBB permeability but also play roles in inflammatory insult after ICH.
