Expressions of IL-6, IL-6 membrane receptor, its soluble IL-6 receptor and soluble glycoprotein 130 in brain tissues of refractory temporal lobe epilepsy
10.3760/cma.j.issn.1671-8925.2012.06.015
- VernacularTitle:难治性颞叶癫痫脑组织IL-6、IL-6受体及sIL-6R、 sgp130的表达及意义
- Author:
Qing YIN
1
;
Hoi-Feng SHU
;
Hong-Liang LIU
;
Hui YANG
;
Ru-Bing YAN
Author Information
1. 第三军医大学附属西南医院
- Keywords:
Temporal lobe epilepsy;
Interleukin-6;
Interleukin-6 receptor;
Soluble interleukin-6 receptor
- From:
Chinese Journal of Neuromedicine
2012;11(6):605-608
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression levels ofinterlenkin-6 (IL-6), IL-6 receptor (IL-6R),its soluble IL-6 receptor (sIL-6R) and soluble glycoprotein 130 (sgp130) in brain tissues of refractory temporal lobe epilepsy (TLE) and explore their role in TLE. Methods Forty patients with TLE and other 40 patients with trauma/hypertensive cerebral hemorrhage (controls), admitted to and performed surgery in our hospital from January 2010 to December 2010,were chosen in our study.Brain tissues from temporal lobe were collected during the surgery.By using real time-PCR and ELISA,the mRNA or protein expression levels of IL-6,IL-6R,sIL-6R and sgp130 were analyzed. Results The mRNA expressions of IL-6 and IL-6R in the TLE brain tissues were significantly higher as compared with those in controls (P＜0.05).ELISA indicated that sIL-6R level in the TLE brain tissues was significantly higher than those in controls (P＜0.05); however,sgp130 level in the TLE brain tissues was slightly higher than those in controls without significant difference (P＞0.05). Conclusion The high level of IL-6 may influence the function of varies types of cells within the epileptic foci of patients with TLE through classical signaling pathway or trans-signaling pathway, which is potentially involved in the epileptogenesis of TLE.
