Influence of hepatocyte growth factor in neovascularization of human glioma
10.3760/cma.j.issn.1671-8925.2012.05.008
- VernacularTitle:HGF对人胶质瘤新生血管形成的影响研究
- Author:
You-Feng GUO
1
;
Meng ZHANG
;
Bing LI
;
Xiao-Zhen JIANG
;
Zhi LI
Author Information
1. 中山大学附属第一医院
- Keywords:
Glioma;
Hepatocyte growth factor;
Neovascularization
- From:
Chinese Journal of Neuromedicine
2012;11(5):469-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect ofhepatoeyte growth factor (HGF) on the neovascularization of human glioma and its mechanism. Methods Seventy-two patients with gliomas,admitted to our hospital from January 2002 to June 2008,including 19 with grade Ⅰ,18 with grade Ⅱ,20 with grade Ⅲ and 15 with grade Ⅳ,were chosen in our study.HGF and CD34 expressions in the paraffin section of gliomas in these 72 patients were detected with immunohistochemistry.Fluorescently labeled HGF-siRNA was transfected into U87MG glioma cells with TurboFect siRNA Transfection Reagent as si-HGF transfection group,and normal control group and negative transfection group were employed as controls; the HGF and vascular endothelial growth factor (VEGF) expressions in these U87MG cells were detected by Western blotting; cells fiom the 3 groups were inoculated to chorioallantoic membrane (CAM), and the angiogenesis ability of these U87MG cells were determined 5 d later. Results HGF-positive rate in patients with low-grade gliomas (grade Ⅰ and Ⅱ) was significantly lower than that in patients with high-grade glioma (grade Ⅲ and Ⅳ,P＜0.05); micro-vascular density (MVD) in gliomas with positive HGF was obviously higher than that in gliomas with negative HGF (P＜0.05).As compared with those in cells of the normal control group and negative transfection group, the HGF and VEGF expressions in cells of the si-HGF transfection group were descended by 76％ and 53％,respectively.The number and area of new vessels and the neovascularization ratio in cells of the si-HGF transfection group were signficantly decreased as compared with those in cells of the normal control group and negative transfection group (P＜0.05). Conclusion Close relation exists between HGF and neovascularization of gliomas,which indicates that HGF might become a new target for future antiangiogenesis treatment.
