Tenuigenin protects the processes of neural stem cells from injury by β-amyloid protein
10.3760/cma.j.issn.1671-8925.2012.03.006
- VernacularTitle:远志总皂苷对Aβ致小鼠神经干细胞突起损伤的保护作用
- Author:
Xiao-Feng ZHU
1
;
Xun-Zhong QI
;
Jing SHI
;
Guang-Tao SUN
;
Jin-Zhou TIAN
Author Information
1. 北京中医药大学东直门医院
- Keywords:
Neural stem cell;
β-amyloid protein;
Tenuigenin;
Scanning electron microscope
- From:
Chinese Journal of Neuromedicine
2012;11(3):242-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect oftenuigenin (TEN) on the processes of neural stem cells (NSCs) injured by β-amyloid (Aβ) protein. Methods Mouse NSCs were generated from the hippocampi of Kunming mice within 24 hour from birth and cultured with epidermal growth factor (EGF)and basic fibroblast growth factor (bFGF) (20 ng/mL each) in 50-mm uncoated culture flasks.The third passage NSCs were cultured in Aβ medium (12.5 μmol/L) and TEN medium (5 mg/L,20 mg/L,100mg/L) respectively and observed under a scanning electron microscope (SEM) after 3 days.Optical microscopy was used to detect the length and amount of the processes of NSCs. The statistical significance between group comparisons was determined by t test.P value ＜0.05 was considered to be statistically significant. Results The length and amount of NSC processes in Aβ1-42 group were both significantly shorter and smaller than in the control group (P＜0.05). The length and amount of NSC processes in 20 mg/L and 100 mg/L groups were both significantly longer and larger than in the Aβ-42group (P＜0.05). Conclusion TEN can significantly increase the length and amount of NSC processes injured by Aβ.
