Effects of nerve growth factor-polybutylcyanoacrylate-nanoparticles on in vitro cellular models of Alzheimer's disease
10.3760/cma.j.issn.1671-8925.2012.03.005
- VernacularTitle:NGF-PBCA-NP的制备与性能评价及其对体外AD细胞模型的保护作用
- Author:
Ying ZHANG
1
;
Chao-Dong ZHANG
;
Jun YANG
;
Jiu-Han ZHAO
;
Xiao-Xue XU
Author Information
1. 中国医科大学附属第一医院
- Keywords:
Nerve growth factor;
Alzheimer's disease;
Polybutylcyanoacrylate-nanoparticles;
PC12
- From:
Chinese Journal of Neuromedicine
2012;11(3):238-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of nerve growth factor (NGF)polybutylcyanoacrylate (PBCA)- nanoparticles (NP) on PC12 cells induced by Aβ1-40 Methods The emulsion polymerization method was used to prepare the NGF-PBCA-NP. Morphology of the NGF-PBCA-NP was observed by transmission electron microscopy. The entrapment efficiency and loading efficiency of NGF-PBCA-NP were measured by means of ultraviolet spectrophotometry. The effects of NGF-PBCA-NP on proliferation and cell apoptosis of β1-40-PC12 cells were observed by MTT and FCM methods. Results The NGF-PBCA-NPs were uniform spheres with drug entrapment efficiency of 80.87％ and loading efficiency of 19.88％.Compared with the cells in the NGF group,cells in the NGF-PBCA-NP group exhibited a significantly better proliferation rate and a significantly lower apoptosis rate (P＜0.05). Conclusion NGF-PBCA-NPs obtained by emulsion polymerization method are uniform in morphology and possess a high drug entrapment efficiency and a high loading efficiency.They can restrain significantly the in vitro growth of Aβ1-40-PC12.
