The oncolytic effect of E1B mutant adenovirus on human malignant gliomas

VernacularTitle:E1B缺陷性腺病毒对人恶性脑胶质瘤的溶瘤作用
Author: Lang-Ping LI ¹ ; Fang-Yi YU ; Jia-Xiang CHEN ; Zhi-Lei XU ; Shao-Yu WU ; Wen-Ya WANG ; Ling L(U) ; Jin-Jun RAO
Author Information

1. 广州市红十字会医院
Keywords: Gliomas; E1B mutant adenovirus; Cytopathic effect
From: Chinese Journal of Neuromedicine 2012;11(3):235-237
CountryChina
Language:Chinese
Abstract: Objective To investigate the oncolytic effect of E1B mutant adenovirus (d11520) on human malignant gliomas. Methods Ad-βgal vector was used to investigate the infectibility of dl1520.U251,Hep3B (positive control) and T24 (negative control) cell lines were infected with dl1520respectively at 50,5,0.5,0.005 and 0 pfu of multiplicity of infection (MOI).The replication efficiency of d11520 in host cells was assessed by plaque assay.The cytopathic effect (CPE) was assessed by crystal violet staining in a panel of tumor cells. Results Crystal violet staining showed the Hep3B cell line was the most sensitive to dl1520 and had the fastest CPE,followed by the U251 cell line,while the T24cell line had no CEP.The replication and infection rates ofdl1520 in the U251 cell line were lower than in the Hep3B cell line but significantly higher than in the T24 cell line (P＜0.05). Conclusion The E1B mutant adenovirus (dl1520) has a significant oncolytic effect on human malignant gliomas.