The effect of Tenascin-R and anti-EGF-L antiserum on the cortical neurons in vitro in rats

VernacularTitle:人TN-R蛋白及其EGF-L片段抗血清对大鼠皮层神经元的影响
Author: Hai-Lin ZHAO ¹ ; Jian YOU ; Guo-Qiang TANG ; Mao-Ying ZHANG ; Xiao-Dan JIANG ; Ru-Xiang XU
Author Information

1. 南方医科大学珠江医院
Keywords: Tenascin-R; Antiserum; Neuron; Axonregeneration
From: Chinese Journal of Neuromedicine 2012;11(3):217-221
CountryChina
Language:Chinese
Abstract: Objective To study the effect of the EGF-L functional region containing human TN-R protein in prokaryocytes and anti-EGF-L serum on the cortical neuronsin vitro in rats. Methods The anti-EGF-L serum was obtained from the rabbits immunized with the protein of EGF-L, then combine with TN-R coat petri dish to prepare various culture substrate.The effect on adhere,migrate and neurite outgrowth of neuron by differ culture substrate was observed. Results High concentration of antiserum against protein of EGF-Lwas successfully obtained from the rabbits,and it has no effect on the neuron when coated on petri dish as culture substrate with PLL in vitro,but can add the adhesive to the neuron,partly neutralize the inhibitory on the neuron neurite of TN-R.The antiserum can also allow the neuron with its neurite to migrate to the area coated with TN-R. Conclusion The anti-EGF-L serum can weaken the inhibitory on the neuron of TN-R in vitro,the function in vivo need further research,