Changes of cerebral ultrastructure and oxidative damage in rats after orthotopic liver autotransplantation
10.3760/cma.j.issn.1671-8925.2012.02.008
- VernacularTitle:大鼠自体原位肝移植后脑组织超微结构的改变及氧化应激特点
- Author:
Hua XIA
1
;
Jun CAI
;
Xin-Jing CHI
;
Ai-Lan ZHANG
Author Information
1. 中山大学附属第三医院
- Keywords:
Liver autotransplantation;
Ultrastructure;
Oxidative damage;
Inflammatory reaction
- From:
Chinese Journal of Neuromedicine
2012;11(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of cerebral ultrastructure,and cerebral oxidative damage and inflammation reaction in rats after orthotopic liver autotransplantation. Methods Twenty-four male SD rats were randomly divided into control group (Group C,n=8),6-h-reperfusion group (Group M1,n=8),and 24-h-reperfusion group (Group M2,n=8).Rats in the Group C received sham operation after anesthesia,involving laparotomy and vascular separation; rats in the Group M1 and M2 received orthotopic liver autotransplantation, and their brain tissues were obtained 6 and 24 h after reperfusion.The changes of cerebral morphology were observed by transmission electron microscope; the levels of interleukin-6 (IL-6),IL-10 and tumor necrosis factor-a (TNF-a) were measured; the levels of superoxide dismutase (SOD),malondialdehyde (MDA) and catalase (CAT) were observed. Results As compared with those in Group C,the cerebral ultrastructure of Group M1 and Group M2 showed obvious traumatic changes,including microvascular edema and deformation,neuron edema,nuclear deformation with indentation in the surface,increment of heterochromatin in the nuclear,blurring in the karyolemma and perinuclear space, disappearance of reticulate structure in the nucleolus,mitochondria edema and disappearance ofendoplasmic reticulum; and these changes in Group M2 were more obvious than those in Group M2.The changes of oxidative damage and inflammatory responses were obvious:as compared with that in Group C, the SOD level in M2 decreased significantly (P＜0.05), the CAT level in M1 decreased significantly (P＜0.05),and the levels of MDA,IL-6,IL-10 and TNF-a in Group M1 increased significantly (P＜0.05). Conclusion After orthotopic liver transplantation,the pathology changes of cerebral tissues in these SD rats were significant, with obvious oxidative damage and inflammatory reaction.
