Effect of profound hypothermia on cytochrome C release and cell apoptosis in the hippocampus after global ischemia in rats
10.3760/cma.j.issn.1671-8925.2012.02.007
- VernacularTitle:深低温对全脑缺血大鼠海马线粒体细胞色素C释放及细胞凋亡影响
- Author:
Hua-Ping QIN
1
;
Chang-Chun YANG
;
Yi ZHANG
;
Tao-Yin XIE
;
Qiang WANG
;
Feng ZHI
;
Ji-Yao JIANG
Author Information
1. 苏州大学附属第三医院
- Keywords:
Profound hypothermia;
Cerebral ischemia;
Cytochrome C;
Cell apoptosis
- From:
Chinese Journal of Neuromedicine
2012;11(2):138-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effect and role of profound hypothermia on cytochrome C release and cell apoptosis in the hippocampus after global ischemia in rats. Methods We established the animal model of cardiopulmonary bypass (CPB) in 24 rats which were randomized into 3 even groups:blank control group,normothermia ischemia group and hypothermia ischemia group.After the hippocampus samples were harvested from the 3 groups,Western blot was used to detect the expression of cytochrome C (Cyt-C) and TUNEL was used to measure the cell apoptosis. Results The expression of Cyt-C in the normothermia ischemia group increased significantly compared with the blank control group but decreased significantly compared with the hypothermia ischemia group (P＜0.05).The rate of cell apoptosis in the normothermia ischemia group increased significantly compared with the blank control group but decreased significantly compared with the hypothermia ischemia group (P＜0.05).Conclusions Profound hypothermia can inhibit cytochrome C release into the cytoplasm and thus reduce cell apoptosis in the hippocampus after global ischemia in rats. This may be one important mechanism underlying the protective effect of profound hypothermia.
