Effect of 17β-estradiol on injured dopaminergic neurons induced by 6-hydroxydopamine
10.3760/cma.j.issn.1671-8925.2011.12.008
- VernacularTitle:17β-雌二醇对6-OH多巴胺损伤的多巴胺能神经细胞的影响
- Author:
Xiao-Zhou WANG
1
;
Ya-Ping LIU
;
Meng WANG
;
Zi-Xiao SHI
;
Xia-Hong XU
;
Ting WANG
;
Dian-Shuai GA0
Author Information
1. 徐州医学院
- Keywords:
Estrogen;
Dopamine;
Tyrosine hydroxylase
- From:
Chinese Journal of Neuromedicine
2011;10(12):1219-1222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of 17β-estrogen(17β-E2)on injured dopaminergic (DA) neurons induced by 6-hydroxydopamine (6-OHDA),and explore whether 17β-E2 has neuroprotective effect.Methods The male SD rats aged 2-3 d were decapitated under sterile conditions; the brain slices were induced injury by 6-OHDA(200 μmol/L); cells in the slices were divided into control group and 17β-E2 treatment groups(intervention with 1.0xl0-8 mol/L,1.0xl0-9 mol/L and 1.0×10-10 mol/L of 17β-E2,respectively).The tyrosine hydroxylase(TH)expression in DA neurons was observed by Western blotting at 15,30 and 60 min after the intervention.Results The expression of TH in the 1.0×10-9 mol/L 17β-E2 treatment group was the highest,which was significantly different as compared with that in the control group(P＜0.05); increasing or decreasing the concentration of 17β-E2 could not regulate the expression of TH; in the 1.0×10-9 mol/L 17β-E2 treatment group,TH expression at 15 min after intervention enjoyed the highest level; the TH expression could not increase by extending the time.Conclusion The 17β-E2 treatment at certain concentration(1.0×10-9 mol/L)for certain time(15 min)has a protective effect on injuried DA neurons
