Effect of salvianolic acid B on activated platelet-induced inflammatory response in human brain micro-vascular endothelial cells
10.3760/cma.j.issn.1671-8925.2011.12.007
- VernacularTitle:丹酚酸B对活化血小板诱导脑微血管内皮细胞炎症应答的影响
- Author:
Shi-Xin XU
1
;
Jun-Ping ZHANG
;
Wei LI
;
Ai-Qin ZHONG
Author Information
1. 天津中医药大学附属第一医院
- Keywords:
Salvianolic acid B;
Activated platelet;
Endothelial cell;
Inflammation
- From:
Chinese Journal of Neuromedicine
2011;10(12):1215-1218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interventional effect ofsalvianolic acid B(SalB)on activated platelet-induced inflammatory response in human brain microvascular endothelial cells (HBMECs).Methods Activated platelets and HBMECs were co-cultured to establish inflammatory response models.The mRNA expression levels of inflammatory mediators,including intercellular adhesion molecule-1(ICAM-1),IL-1β,IL-6,IL-8 and monocyte chemoattractant protein-1(MCP-1)in HBMECs,were detected by quantitative RT-PCR.Cells of the pretreatment group were pretreated with 10 μg/ml SalB for 24 h,and the changes of mRNA expression levels of these inflammatory mediators were analyzed.Results The mRNA expression levels ofICAM-1,IL-1β,IL-6,IL-8 and MCP-1 in activated platelet-induced inflammatory response model of SalB pretreatnent group were significantly reduced as compared with those without pretreatment(P＜0.05).Conclusion SalB can repress the activated platelet-induced inflammatory response in HBMECs,which might be one of the mechanisms of anti-cerebral ischemia effect.
