Aging-related sensibility of dopaminergic neurons to rotenone
10.3760/cma.j.issn.1671-8925.2011.11.006
- VernacularTitle:多巴胺能神经元对鱼藤酮敏感性的增龄改变及其机制
- Author:
Li-Dan CAO
1
;
Ya-Ping YANG
;
Hui-Fen ZHENG
;
Yu HONG
;
Guo-Xiu KE
;
Li-Fang HU
;
Chun-Feng LIU
Author Information
1. 苏州大学附属第二医院
- Keywords:
Parkinson's disease;
Aging;
Rotenone;
Oxidative stress;
Caspase-3
- From:
Chinese Journal of Neuromedicine
2011;10(11):1101-1105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of rotenone neurotoxin on dopaminergic neurons from SD rats of different months and its mechanism.Methods Forty 3-month-old,4012-month-old and 40 20-month-old SD rats were chosen in our study and equally randomized into control group and rotenone treatment group,respectively(n=20); rotenone(1.0 mg/[kg·d])was given to the rats in the rotenone treatment group for 30 consecutive d(having drug withdrawal 1 d per week)to induce rotenone neurotoxin models.Tyrosine hydroxylase immunoreactivity in the substantia nigra pars compacta(SNpc)was observed.Levels of total superoxide dismutase(SOD)and catalase(CAT)were determined by colorimetric method and protein level of caspase-3 was determined by Western blotting.Results A significant decreased number of TH-positive cells in 3-,12-and 20-month-old rats of rotenone treatment group was noted as compared with that in the control group,respectively(P＜0.05),and 20-month-old rats had the fewest number.SOD and CAT activities in 3 month-old rats of the control group enjoyed the highest level and decreased trend was noted in 20 month-old rats of the control group;as compared with those in the control group,the SOD and CAT activities in all the rats of rotenone treatment group increased significantly(P＜0.05).Western blotting revealed that the expression of cleaved caspase-3 in all the rats of rotenone treatment group obviously increased as compared with those in the control group,and 20-month-old rats showed the highest level of active caspase-3.Conclusion Rotenone can induce the decrease of TH-positive cell level following the increase of rat age; increase levels ofcaspase-3 and oxidative stress may take part in the mechanism of high sensibility of old SD rats to rotenone.
