Relation between recurrent hemorrhage and both ultrastructural features of vascular and expressions of angiopoietin 1,CD68 and matrix metalloproteinase-9 in intracranial cavernous hemangioma
10.3760/cma.j.issn.1671-8925.2011.10.012
- VernacularTitle:颅内海绵状血管瘤超微结构特征及Ang-1、CD68、MMP-9表达与其反复出血的关系
- Author:
Chun-Guang CHEN
1
;
Ning SUN
;
Jun-Hong GUAN
Author Information
1. 辽宁省辽阳市中心医院
- Keywords:
Intracranial cavernous hemangioma;
Hemorrhage;
Matrix metalloproteinase-9;
CD68;
Angiopoietin 1
- From:
Chinese Journal of Neuromedicine
2011;10(10):1019-1022
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relation between recurrent hemorrhage and both ultrastructural features of vascular and expressions of angiopoietin 1 (Ang-l),CD68 and matrix metalloproteinase-9 (MMP-9) in intracranial cavernous hemangioma.Methods Thirty-five intracranial cavernous hemangioma samples,35 hepatic cavernous malformation samples and 4 normal brain tissues were chosen in our study; the ultrastructural features of vascular were observed with optical microscope and electron microscope; immunohistochemical methods were used to analyze the expressions of Ang-1,CD68 and MMP-9.Results The vascular wall was very thin in intracranial eavemous hemangioma samples,only with thin monolayer of endothelial cells and adventitia,but without basal lamina and elastic layer; gap junctions were obviously observed between endothelial cells; basement membrane showed unintelligible,and a large number of high electron density of sediment was presented at the surrounding blood vessels.Tissues from intracranial cavernous hemangioma obviously expressed higher levels of CD68 and MMP-9 than the normal brain tissues (P＜0.05); Rank correlation analysis indicated that the expressions of CD68 and MMP-9 had a high degree of positive correlation (r=0.870,P=0.000).Tissues from intracranial cavernous hemangioma expressed less Ang-1 than normal brain tissues without significant difference (P＞0.05).Conclusion The vascular structural and functional defection and instability in patients with cavernous hemangioma provide favorable conditions for the happaning of recurrent hemorrhage; and high expressions of CD68 and MMP-9 are closely related with recurrent hemorrhage.
