Neuroprotective effect of Chinese tarantula Chilobrachys jingzhao venom on cerebral ischemiareperfusion injury in mice
10.3760/cma.j.issn.1671-8925.2011.10.009
- VernacularTitle:敬钊缨毛蛛毒素对小鼠脑缺血再灌注损伤神经保护作用
- Author:
Xi-Yang MI
1
;
Xiao-Jun SHI
;
Wu-Lin WANG
Author Information
1. 清华大学
- Keywords:
Spider toxin;
Cerebral ischemia;
Superoxide dlsmutase;
Malonaldehyde;
Cyclooxygenase-2
- From:
Chinese Journal of Neuromedicine
2011;10(10):1005-1008
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect ofJingzhaotoxin (JZTX) from Chinese tarantula Chilobrachys jingzhao venom on cerebral ischemia/reperfusion injury in mice.Methods Twenty mice were equally randomized into normal group,sham-operated group,vehicle group and JZTX treatment group (n=5).Cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion.Cerebral infarct volume was measured by TTC staining.The superoxide dlsmutase (SOD) activity and malonaldehyde (MDA) content in serum were detected with colorimetric method.Immunohistochemistry and real-time PCR were used to analyze the expressions of cyclooxygenase-2 (COX-2).Results The cerebral infarct volume in the JZTX treatment group was significantly decreased as compared with that in the vehicle group (P＜0.05); higher SOD activity and lower MDA content in the JZTX treatment group after ischemic insult were noted than those in the vehicle group (P＜0.05); the mRNA and protein expressions of COX-2 in the JZTX treatment group was significantly down-regulated as compared with those in the vehicle group (P＜0.05).Conclusion JZTX has neuroprotective effect in cerebral ischemia/reperfusion injury,whose mechanism might be related to the improvement of antioxidant capacity and the down-regulation of COX-2 expressions after cerebral ischemia.
