Influence of mild hypothermia on apoptotic protease activating factor-1 protein expression in the hippocampal CA1 area and learning and memory functions of rats after diffuse brain injury
10.3760/cma.j.issn.1671-8925.2011.07.006
- VernacularTitle:亚低温对大鼠弥漫性脑损伤后海马CA1区Apaf-1蛋白表达和学习记忆功能的影响
- Author:
Peng YANG
1
;
Yun LI
;
Jun ZHU
;
Qing-Jun LIU
;
Ai-Jun FU
;
Tong CHEN
;
Li-Min ZHANG
;
Xi-Feng ZOU
;
Ze-Lin SUN
;
Qun-Xi LI
;
Yun-He ZHANG
Author Information
1. 河北联合大学附属医院
- Keywords:
Craniocerebral trauma;
Hippocampus;
Apoptotic protease activating factor-1;
Mild hypothermia
- From:
Chinese Journal of Neuromedicine
2011;10(7):669-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of mild hypothermia on the apoptotic protease activating factor-1 (Apaf-1) protein expression in the hippocampal CA1 area and learning and memory functions of rats after diffuse brain injury (DBI). Methods Forty-eight male Wistar rats were equally randomized into normal group, sham-operated group, brain injury group and mild hypothermia treatment group (n=12). Rats in the normal group did not receive any treatment; rats in the sham-operated group only received anesthesia, and incision and suture of scalp; rats in the brain injury group and mild hypothermia treatment group were induced the DBI models according to Marmarou method; and rats in the mild hypothermia treatment group were performed hypothermia with ice blanket and ice bag to control the rectal temperature within 30.0-31.0 ℃ for 2 h. After DBI for 2 weeks, the times of searching refuge platform and the protein expression of Apaf-1 were compared among the 4 groups. Results The times of searching refuge platform in the normal group, sham operated group, brain injury group and mild hypothermia treatment group were (10.1 ±1.9), (10.3±1.8), (3.8±2.3) and (6.9±1.1), respectively,with significant differences between each 2 groups (P<0.05). The protein expression of Apaf-1 in the brain injury group and mild hypothermia treatment group was obviously higher than that in the other groups (P<0.05), and significantly lower protein expression of Apaf-1 in the mild hypothermia rats was noted as compared with that in the brain injury rats (P<0.05). Conclusion Mild hypothermia might play a protective role by inhibiting neuronal apoptosis for rats with DBI through the Apaf-1 pathway,which can improve learning and memory abilities.
