Effects of cyclophilin A on apoptosis of PC12 cells induced by Aβ25-35 and its mechanism
10.3760/cma.j.issn.1671-8925.2011.06.011
- VernacularTitle:亲环素A对Aβ25-35诱导PC12细胞凋亡的影响及机制研究
- Author:
Yu-Song GE
1
;
Lin YIN
;
Wei-Yu TENG
;
Chao-Dong ZHANG
Author Information
1. 大连医科大学附属第二医院
- Keywords:
Cyclophilin A;
Aβ25-35;
Apoptosis;
Bcl-2;
Bax
- From:
Chinese Journal of Neuromedicine
2011;10(6):582-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of cyclophilin A (CyPA) on apoptosis of PC 12 cells induced by Aβ25-35 and its potential mechanism. Methods PC 12 cells were divided into normal control group (0 μmol/L Aβ25-35), Aβ25-35 inducement group (10 μmol/L Aβ25-35) and drug protection groups (0.1, 1,10 and 100 nmol/L CyPA+10 μmol/L Aβ25-35). Cells in the drug protection groups were pretreated by CyPA of different concentrations for 30 min, and then co-cultured with Aβ25-35 We evaluated the survival rate of PC12 cells with MTT assay, analyzed the apoptosis of PC12 cells with Hoechst33258 staining, and detected the mRNA expressions of Bcl-2 and Bax with PT-PCR and the protein levels of Bcl-2 and Bax with Western blotting. Results Cells pretreated wth CyPA of 1, 10 and 100 nmol/L enjoyed an obvious elevation of survival rate of PC 12 cells, a significant reduction of apoptosis induced by Aβ25-35,an obvious increase of mRNA expression of Bcl-2 and protein level of Bcl-2, and a statistical decrease of mRNA expression of Bax and protein level of Bax as compared with those cells of the Aβ25-35 inducement group (P<0.05);and these effects were dose-dependent. Conclusion CyPA could resist the toxic role of Aβ25-35 on PC 12 cells and reduce the apoptosis in a dose-dependent manner by up-regulation of anti-apoptosis gene Bcl-2 and down-regulation of apoptosis gene Bax.
