Immuno-pathogenesis of B7-H1 in polymyositis
10.3760/cma.j.issn.1671-8925.2011.05.022
- VernacularTitle:B7-H1分子免疫病理在多发性肌炎中的临床研究
- Author:
Qi FANG
1
;
Yun-Xia WANG
;
Xiu-Ying CAI
;
Jian-Hua JIANG
;
Xiao-Yu DUAN
Author Information
1. 苏州大学附属第一医院
- Keywords:
Polymyositis;
Immunohistochemistry;
Costimulatory molecule
- From:
Chinese Journal of Neuromedicine
2011;10(5):516-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the protein expression of costimulatory molecule B7-H1 in muscular tissues of patients with polymyositis (PM) and limb-girdle muscular dystrophy-2B type (LGMD-2B), and investigate its relevance to the pathogenesis of PM and its role in the diagnosis and identification of PM. Methods Forty-three patients with PM, 26 patients with LGMD -2B and 21 with normal muscle biopsy were recruited. Muscle biopsy was performed before frozen sections, and then, HE staining and immunohistochemistry were employed to detect the protein expression of B7-H1 in muscle tissues of each group. Results The results of HE staining of muscle tissues in the PM group and LGMD 2B group were very similar; varying degrees of necrosis, phagocytosis and regeneration phenomenon were noted with varying degrees of inflammatory cell infiltration. In PM group,muscle-related expression of B7-H1 was observed on the surface of muscle fibers (the cytomembrane). It was localized in areas where inflammatory cells lay in close apposition to damaged or non-necrotic muscle fibers. The B7-H1 protein in the PM muscular tissue was significantly increased as compared with that in the LGMD -2B tissue and normal tissue (69.77%, 26.92%, 4.76%, P<0.05). Conclusion Costimulatory molecule B7-H1 is highly expressed in the muscular tissue of patients with PM and it may be involved in the immunological pathogenesis of PM. It can be used to make a distinction between PM and other myopathies that have secondary inflammatory changes.
