Culturing Schwann cells by explants and modified enzyme digestion technique

VernacularTitle:改良的酶消化后植块法体外培养雪旺细胞
Author: Hui WANG ¹ ; Lun LUO ; Wen-Sheng LI ; Chao-Feng LIANG ; Hai-Yong HE ; Ying GUO
Author Information

1. 中山大学附属第三医院
Keywords: Schwann cell; Cell culture technology; Enzyme digestion; Explant
From: Chinese Journal of Neuromedicine 2011;10(5):461-463
CountryChina
Language:Chinese
Abstract: Objective To introduce an efficient method for culturing and purifying Schwann cells (SCs) in vitro. Methods Sciatic nerves were harvested from neonatal SD rats and the epineuria were removed. Single enzyme digestion combined with explans and double enzyme digestion were employed, respectively, to digest the nerve tissues following trituration. The proliferation of SCs and degree of purification were evaluated by viable count method and the combination of S-100 labeled monoclonal antibody and SCs. Results Proximally 3.5 × 106 cells were harvested with 96% survival rate and a purity of Schwann cells over 94% by single enzyme digestion combined with explans, however only 3.0×106 cells were gained with the purity being 90% and survival rate being 92% by double enzyme digestion. Conclusion This method yields large amount of viable Schwann cells with high purity and survival rate.