Relationship between Rac1 expression of human gliomas and their pathological grades

VernacularTitle:Rac1在人神经胶质瘤组织中的表达及其与病理分级的相关性研究
Author: Yan-Xia MA ¹ ; Yi-Quan KE ; Zhi-Lin YANG ; Shen-Tao ZHOU ; Xi-Feng LI ; Xin-De ZHAO
Author Information

1. 南方医科大学珠江医院
Keywords: Glioma; Rac1; Immunofluorescence; PCR; Western blotting
From: Chinese Journal of Neuromedicine 2011;10(4):373-376
CountryChina
Language:Chinese
Abstract: Objective To investigate the protein and mRNA levels of Rac1 in glioma tissues,and explore the correlation of Rac1 with pathological grades. Methods Immunofluorescence,RT-PCR and Western blotting were used to detect the protein and mRNA levels of Rac1 in 45 cases of gliomas tissues and 10 cases of normal brain tissues. Results The results indicated that normal brain tissues showed no protein and mRNA expressions of Rac1, and that of Rac1 highly expressed in glioma tissues (42/45 at most). The spearman correlation analysis revealed that the levels of transcription and expression of Rac1 were positively correlated to the tumor grades; positive expression rate of Rac1 in high grade of glioma was statistically higher than that in low grade of glioma. Conclusion The high expressions of Rac1 in the glioma are closely correlated to the tumor cell invasion and metastasis, which can be used as a marker indicating the malignance and proliferation of glioma.