Association between polymorphisms of CD28/ICOS gene in chromosome 2q33 region and both susceptibility to and severity of multiple sclerosis in Han people of south China
10.3760/cma.j.issn.1671-8925.2011.03.008
- VernacularTitle:2q33区段CD28/ICOS基因多态性与中国南方汉族人群多发性硬化疾病易感性及严重程度关系的研究
- Author:
Yu-Zhen CUI
1
;
Cheng-Hong LUO
;
Bing-Ru SU
;
Bo XIAO
;
Wen-Bing ZHOU
Author Information
1. 东莞市石龙人民医院
- Keywords:
Multiple Sclerosis;
Polymorphism;
Mononucleotide;
Polymerase chain reaction
- From:
Chinese Journal of Neuromedicine
2011;10(3):246-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the polymorphisms of CD28 and COS genes in chromosome 2q33 region and susceptibility to and severity of multiple sclerosis (MS).Methods Eighty-three patients diagnosed as having MS from Han population of South China were studied; one hundred and ten patients with non-autoimmune diseases or healthy volunteers were selected as controls.DNA was obtained from peripheral venous blood; the polymorphisms of amplified productions (CD28 and ICOS genes) were detected by PCR-RFLP and analyzed by agarose gel electrophoresis.Immunofluorescent two-color flow cytometry was used to study the expressions of CD28 and ICOS genes of lymphocytes in the peripheral blood of these 2 groups. Results The genotype frequency of TT at ICOS-2394 site in patients with MS (33.7%) was significantly higher than that in controls (10.9%,P＜ 0.05), and the allele frequency of T in patient group (56.0%) was obviously higher than that in the controls (30.9%, P＜0.05). No marked combined effects were noted in the 3 SNPs (CD28-372, ICOS-2349 and ICOS-2119). TT genotype in the polymorphic site of ICOS-2394 was correlated to primary progressive MS, while CT genotype in the polymorphic site of ICOS-2394 was not correlated to secondary progressive MS. The genotype and allele frequencies of the 3 SNPs had no marked association with the severity of MS (P＞0.05). The levels of CD28 and ICOS gene expressions in lymphocytes of peripheral blood of patients with MS were significantly higher in patients than that in control group (P ＜ 0.05). Conclusion ICOS polymorphisms might be related to MS in Han population of South China,which suggests that ICOS might be one of genes having susceptibility to MS. No association between CD28 gene polymorphisms and susceptibility to MS is noted.
