Hyperbaric oxygen therapy in rabbits after explosive brain injury: a MRS and DWI study
10.3760/cma.j.issn.1671-8925.2011.01.008
- VernacularTitle:MRS联合DWI观察实验性兔颅脑爆震伤后高压氧的治疗作用
- Author:
Yan-Yan YANG
1
;
Jia-Chuan LIU
;
Yong-Ming ZHANG
;
Wen-Jiang SUN
;
Hong TANG
;
Zhen-Shan HUANG
;
Bing-Cang LI
;
Liang-Chao ZHANG
Author Information
1. 解放军第105医院
- Keywords:
Brain injury;
Blast injury;
Magnetic resonance spectroscopy;
,Diffusion-weighted MRI;
Hyperbaric oxygen
- From:
Chinese Journal of Neuromedicine
2011;10(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of magnetic resonance spectroscopy (MRS) and diffusion-weighted MRI (DWI) in detecting regional cerebral metabolic changes and changes of water molecular motion in rabbits after explosive brain injury at different time points of injury after being treated with hyperbaric oxygen therapy. Methods Ninety New Zealand white rabbits were divided into control group, trauma group and hyperbaric oxygen treatment group. The injured models in the later 2 groups were established using 600 mg TNT equivalent of paper detonators in the rabbit brain at the top of 6.5 cm vertical distance. Rabbits in the trauma group and hyperbaric oxygen treatment group were sub-divided into 1, 3, 7, 14 and 21 d treatment groups, respectively. The survival of these rabbits was observed at these time points. Hyperbaric oxygen treatments lasting for 1, 3, 7, 14 and 21 d were given to each hyperbaric oxygen treatment sub-groups, respectively. Performance under MRS was detected and dynamic changes of N-acetylaspartate (NAA)/creatine (Cr) ratio and NAA/choline(Cho)+Cr value were observed with MRS. DWI was employed to detect the dynamic changes of apparent diffusion coefficient (ADC) values. Results The NAA/Cr ratio in the trauma group markedly decreased right after the injury and slightly rose 7 d after the injury. Compared with that in the trauma group, the NAA/Cr ratio in the hyperbaric oxygen treatment group was significantly increased (P＜0.05). The NAA/Cho+Cr value in the trauma group was markedly decreased right after the injury, while that in the hyperbaric oxygen treatment group was obviously increased as compared with that in the trauma group (P＜0.05). ADC values in the region of interest of the trauma group was decreased after 1 d of treatment and gradually increased after 3 d of treatment; the ADC values in the hyperbaric oxygen treatment group was obviously higher than those in the trauma group (P＜0.05). Conclusion Hyperbaric oxygen might improve the prognosis by improving local metabolism of neurons, inhibiting brain edema, and enhancing local gliosis repair;, ultra-early intervention (within one week of injury) may be much favorable for animals/patients after explosive brain injury.
