Expressions of perforin and granzyme-B in CD8+ memory T cell subsets in patients with multiple sclerosis
10.3760/cma.j.issn.1671-8925.2010.12.009
- VernacularTitle:多发性硬化患者CD8+记忆性T细胞亚群穿孔素和颗粒酶-B表达的研究
- Author:
Guang-Zhi LIU
1
;
Chao WANG
;
Yang HE
;
Yu-Juan JIAO
;
Xu-Guang GAO
Author Information
1. 北京大学人民医院
- Keywords:
Multiple sclerosis;
CD8-positive T-lymphocyte;
Granzyme-B;
Perforin
- From:
Chinese Journal of Neuromedicine
2010;09(12):1221-1224
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the expressions of cytotoxity-related cytokines in CD8+memory T cell subsets of peripheral blood in patients with multiple sclerosis (MS) and further analyze the relationship between the cytokine levels and MS disease severity. Methods Four-color flow cytometry was employed to detect the proportion of CD8+ memory T cell subsets expressed perforin and granzyme-B in peripheral blood of patients with MS, with other neurological disorders (OND) and of normal control (NC) group. The expanded disability status scale (EDSS) was used to reflect the disease severity at the time of blood sampling. Results Patients with MS had a decreased proportion of effector memory CD8+ T (CD8+ TEM) cells and terminal TEM cells expressed granzyme-B as compared with NC group (P＜0.05). CD8+ TEM cells expressed granzyme-B and perforin were negatively correlated to the scores of EDSS in patients with MS (r=-0.493, P=0.027; r=-0.594, P=0.009). Conclusions CD8+ TEM may be involved in the MS-related CNS inflammatory immune response. The proportion of CD8+ TEM cells expressed perforin and granzyme-B can be used to reflect the pathological severity of MS lesions to some degrees.
