Intervention effect of the minimally invasive hematoma aspiration on repair of pyramidal tract in rat models after basal ganglia hemorrhage
10.3760/cma.j.issn.1671-8925.2010.12.007
- VernacularTitle:微创血肿清除治疗对基底节脑出血大鼠锥体束的影响
- Author:
Bi-Song LIU
1
;
Zhao-Cong ZHENG
;
Ru-Mi WANG
Author Information
1. 南京军区福州总医院
- Keywords:
Intracerebral hemorrhage;
Pyramidal tract;
Minimally invasive hematoma aspiration
- From:
Chinese Journal of Neuromedicine
2010;09(12):1212-1216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of the minimally invasive hematoma aspiration on the repair of the pyramidal tract and improvement of neurological function. Methods Forty-eight SD male rats were equally randomized into normal control group, sham-operated group, cerebral hemorrhage group (model), model + minimally invasive hematoma aspiration at the 6th, 12th, and 24th h groups (n=8).Intracerebral hemorrhage in the later 4 groups was induced by injection of type IV collagenase + heparin into the caudate nucleus of rats, and the same amount of normal saline was injected into the sham-operated group. And then, the hematoma was lysed by injection of urokinase into the hematoma center 6, 12 and 24 h after intracerebral hemorrhage in the later 3 groups, respectively. Animals were sacrificed after behavioral function evaluation 14 d after collagenase injection. Immunohistochemistry was performed to observe the expressions of neurofilament (NF) and growth associated protein-43(GAP-43) in the posterior limb of internal capsule. Results The snatch ability of left forelimb among all the groups showed no significant differences before the success of model making (P＞0.05). The snatch ability of left forelimb in each hematoma aspiration group was much higher than that in the model group on the 14th d of collagenase injection (P＜0.05). Within the hematoma aspiration groups, the snatch ability in the group performed hematoma aspiration at the 6th h was higher than those group at the 12th and 24th h (P＜0.05). The number of NF positive fibers and the expression of GAP-43 in the 3hematoma aspiration groups were much larger or higher than those in the model group (P＜0.05); the number of NF positive fibers in the group performed hematoma aspiration at the 6th h was larger than that in those group at the 12th and 24th h (P ＜0.05); The expression of GAP-43 in the group performed hematoma aspiration at the 6th and 12th h was higher than that in the group at the 24th h (P＜0.05).Conclusion The minimally invasive hematoma aspiration performed within 24 h of intracerebral hemorrhage, especially those within 6 h, would reduce the pyramidal tract's injury, promote the repair of pyramidal tract and improve the neurological function.
