Study on development of DNA probe for identification of Prevotella intermedia G8-9K-3.
10.5051/jkape.2002.32.2.281
- Author:
Jong Sung BAK
1
;
Se Hoon KIM
;
Dong Kie KIM
;
Jin Hyo SEONG
;
Byung Ock KIM
;
Mi Kwang KIM
;
Joong Ki KOOK
Author Information
1. Department of Periodontology, College of Dentistry, Chosun University, Korea.
- Publication Type:Original Article
- Keywords:
Prevotella intermedia G8-9K-3;
DNA probe
- MeSH:
Base Sequence;
Blotting, Southern;
DNA*;
Gene Library;
Mass Screening;
Plasmids;
Prevotella intermedia*;
Prevotella*
- From:The Journal of the Korean Academy of Periodontology
2002;32(2):281-290
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study is to develop species-specific DNA probe for detection and identification of Prevotella intermedia (P. intermedia) G8-9K-3. This study procedure includes (1) whole-genomic DNA extraction of P. intermedia G8-9K-3 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot hybridization, (4) confirmation of strain-specific DNA probe by Southern blot hybridization, (5) determination of nucleotide sequences of strain-specific DNA probe. Twenty-eight recombinant plasmids containing Hind III-digested DNA fragments of P. intermedia G8-9K-3 were obtained. Reverse dot Hybridization and Southern blot analysis data showed that one of them, Pig3, could be P. intermedia G8-9K-3-specific DNA probe. This datum indicates that this Pig3 DNA probe could be useful in detection and identification of the P. intermedia G8-9K-3 strain.
