Differentially expressed genes in the hippocampus of rats with global ischemia : an Affymetrix microarray study

VernacularTitle:利用Affymetrix芯片筛选全脑缺血大鼠海马差异表达基因
Author: Hua-Ping QIN ¹ ; Ju-Hua ZHANG ; Yi-Lin YANG ; Yi-Min SHI ; Jian-Jun ZHOU ; Wei GUAN ; Ji-Yao JIANG
Author Information

1. 苏州大学附属第三医院
Keywords: Global cerebral ischemia; Gene chip; Differentially expressed gene
From: Chinese Journal of Neuromedicine 2010;09(11):1095-1098
CountryChina
Language:Chinese
Abstract: Objective To screen the differentially expressed genes in the hippocampus of rats with global ischemia. Methods We established the animal models of cardiopulmonary bypass (CPB)in rats, and the rats were divided into 2 groups: rats in control group (n=3) were killed 5 minutes after CPB and rats in ischemia group (n=3) experienced 5-minutes' circulatory arrest. The hippocampal tissue was got from the 2 groups. Affymetrix ratgenome 230 2.0 array was used to detect the gene expression profile of each hippocampus in the 2 groups and the differentially expressed genes were analyzed.Results Fifty-five transcripts (30, up-regulated; 25, down-regulated) in the ischemia group changed; 28transcripts of them changed significantly as compared with those in the control group (P＜0.05).Conclusion Screening the differentially expressed genes may be important in elucidating the molecular biological mechanism of global cerebral ischemic injury.