Effect of collagen scaffold loaded with collagen-targeting neurotrophin-3 on the extension of cellular processes of dorsal root ganglion
10.3760/cma.j.issn.1671-8925.2010.10.011
- VernacularTitle:有序胶原支架联合胶原靶向神经营养因子3对背根节细胞突起延伸的影响
- Author:
Guo-Qiang TANG
1
;
Ru-Xiang XU
;
Juan FAN
;
Xiao-Dan JIANG
;
Sha XUE
;
Jian YOU
;
Ying-Qian CAI
Author Information
1. 南方医科大学珠江医院
- Keywords:
Collagen;
Scaffold;
Neurotrophin-3;
Spinal cord injury
- From:
Chinese Journal of Neuromedicine
2010;09(10):1009-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of collagen scaffold loaded with collagen-binding domain neurotrophin-3 (CBD-NT3) on the extension of cellular processes of dorsal root ganglions (DRGs), and explore the significance of this kind of combinatorial strategies in the spinal cord injury repair. Methods The tail tendons of SD neonatal rats were performed the removal of cellular components to prepare the collagen scaffold; HE staining was employed to evaluate whether the cells were completely removed from the collagen scaffold. The collagen scaffold was loaded with CBD-NT3,and then, they were co-cultured with primary DRGs for 1, 3 and 5 d, respectively. NT3 and PBS were also co-cultured with primary DRGs for 1, 3 and 5 d, respectively, as controls. Cells on the scaffold were stained by fluorescein diacetate (FDA) for morphology observation and the lengths and angles of the processes in each group were also quantitatively analyzed. Scanning electron microcopy (SEM) was employed to observe the topography of scaffold and the ultrastructure of DRGs 3 d after the co-culture.Results HE staining indicated that the cellular components in the scaffold were removed completely.The length of processes elongation in CBD-NT3 treatment group was significantly longer than that in the controls 3 d after the co-culture (P＜0.05). The 95% confidence interval of the angle between the line which the process emerged from the cell soma to the growing tip of the process and the long axis of fiber was 18.8-20.7 degrees. The results of SEM showed that cells could rely on the topography of the scaffold to anchor and grow. Conclusion The combinatorial strategies of collagen scaffold with CBD-NT3 can play a double function for oriented guiding and inducing extension of cellular processes effectively,which may provide a better therapeutic approach for spinal cord injury repair.
