Adipose-derived stem cell transplantation on motor function of ischemic rats
10.3760/cma.j.issn.1671-8925.2010.10.010
- VernacularTitle:脂肪来源干细胞移植对脑缺血大鼠运动功能的影响
- Author:
Xue-Qian YUAN
1
;
Li-Feng ZHANG
;
Huan-Rong WANG
;
Tai-Qing ZHU
Author Information
1. 郑州人民医院
- Keywords:
Stem cell transplantation;
Cerebral ischemia;
Immunofluorescence
- From:
Chinese Journal of Neuromedicine
2010;09(10):1005-1008
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the survival, migration and differentiation of adipose-derived adult stem (ADAS) cells and the recovery of motor function of ischemic rats after ADAS cell transplantation. Methods Eighteen adult male SD rats, weighted about 250-300 g, were chosen and received left middle cerebral artery occlusion (MCAO) operation. And then, they were equally randomized into untreated group, control group and ADAS cell transplantation group. Tail vein injection of Dulbecco's modified eagles medium (DMEM) and ADAS cells were performed in the control group and ADAS cell transplantation group 3 h after MCAO, respectively. These animals were euthanized 14 d after MCAO. Immunofluorescence for BrdU, NSE, MAP-2 and GFAP were processed to identify the survival, migration and differentiation of grafted ADAS cells in the brain, and at the same time, scores of neurological deficit scale were used to assess the improvement of motor function. Results After the transplantation, numerous ADAS cells labeled with BrdU were presented in the ischemic points and surrounding areas. A few BrdU/GFAP, BrdU/NSE and BrdU/MAP-2 -positive cells were noted in the ischemic points of ADAS transplantation group 14 d after MCAO. The neurological functional recovery in the ADAS cell transplantation group was significantly improved as compared with that in the control group 14 d after MCAO (P＜0.05). Conclusion ADAS cells can migrate into the ischemic hemisphere and differentiate into neuron-like and astrocytic-like cells after the injection by venous approach in the rat models with MCAO. The intravenous administration of ADAS cells into rats with MCAO leads to good functional outcome and few lesion sizes.
