Effect of human urinary kallidinogenase on spatial learning and memory functions and nestin expression in peri-infarction cortex of rats after focal cerebral ischemia-reperfusion
10.3760/cma.j.issn.1671-8925.2010.10.009
- VernacularTitle:人尿激肽原酶对大鼠脑缺血再灌注后空间学习记忆功能及皮质巢蛋白表达的影响
- Author:
Lei FAN
1
;
Jin-Sheng YANG
;
Xiang-Qun SHI
Author Information
1. 兰州军区兰州总医院
- Keywords:
Human urinary kallidinogenase;
Cerebral ischemia-reperfusion;
Spatial learning and memory
- From:
Chinese Journal of Neuromedicine
2010;09(10):1001-1004
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human urinary kallidinogenase (HUK) on the abilities of spatial learning and memory and the expression of nestin in the peri-infarction cortex of rats after focal cerebral ischemia-reperfusion. Methods Sixty rats were equally randomized into 5 groups:sham-operated group, model group, low dose HUK treatment group (3.5 ×10-3 PNAU/kg), median dose HUK treatment group (8.75×10-3 PNAU/kg) and high dose HUK treatment group (17.5×10-3 PNAU/kg).The focal cerebral ischemia-reperfusion models in the model group and HUK treatment groups were established by introducing an intraluminal filament into the right middle cerebral artery of the rats. HUK was administered intraperitoneally right after the operation and afterward once daily for 2 weeks. The spatial learning and memory functions were studied by Morris water maze test, and the nestin expression in the peri-infarction cortex was measured by immunohistochemistry on the 15th d. Results The model group exhibited seriously spatial learning and memory deficits in both place navigation trail and spatial probe trial. In the place navigation trial, the mean values of escape latency in the median dose and high dose HUK treatment groups were shorter than those in the model group (P＜0.05). In the spatial probe trial, significant differences in the percentages of time spending in the former platform quadrant and frequency of crossing the former platform site were noted between the model group and both median dose and high dose HUK treatment groups (P＜0.05). Immunohistochemical analysis showed that the nestin expression in the peri-infarction cortex of median dose and high dose HUK treatment groups increased significantly as compared with that in the model group (P＜0.05). Conclusion Treatment with HUK improves the spatial learning and memory abilities in rats after focal cerebral ischemia-reperfusion, which may result from the increasing expression ofnestin and the proliferation of neural stem cells.
