Microglias play a dual role in the induction of cerebral ischemic tolerance
10.3760/cma.j.issn.1671-8925.2010.09.009
- VernacularTitle:小胶质细胞活化在脑缺血耐受中的双重作用
- Author:
Yu-Man HAO
1
;
Zu-Ming LUO
;
Dong ZHOU
;
Li GAO
;
Zhong ZHANG
;
Zhong ZENG
;
Yan LIU
;
Fu-Wei SHEN
Author Information
1. 重庆医科大学附属成都第二临床学院
- Keywords:
Ischemic preconditioning;
Ischemic tolerance;
Cerebral infarction;
Microglia
- From:
Chinese Journal of Neuromedicine
2010;09(9):901-904
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of focal cerebral ischemic preconditioning (IPC)on the activation of microglias to explore the endogenous neuroprotection of microglias. Methods SD rats were divided into 4 groups, in which the IPC (10 minutes)+MCAO group and SS (without IPC)+MCAO group received middle cerebral artery occlusion (MCAO, 2 h) followed by reperfusion (22 h),and the other 2 groups received SS+SS (blank controls) or IPC+SS (without MCAO). The infarct volume,ultrastructure and activation changes of microglias were evaluated in each group by riphenyltetrazolium chloride (TTC) staining, electron microscope and immunohistochemical staining, respectively. Results Compared with SS+MCAO group, the further reduction of infarct volume and slight ultrastructure changes were observed in the IPC+MCAO group. More severe pathological changes in the SS+MCAO group were observed than those in the IPC+MCAO group. In contrast with that in the IPC+SS group, the activation of microglias further increased in the SS+MCAO and IPC+MCAO group; while that in the SS+MCAO was significantly higher than that in the IPC+MCAO group (P＜0.05). Conclusion Microglias play a dual role in the induction of ischemic tolerance, and inflammation mediated by activation of microglias might be a key event in the mechanism of endogenous neuroprotection induced by focal IPC.
