Protective effect of PEP-1-SOD1 pretreatment on parietal cortex of mice with cerebral infarction
10.3760/cma.j.issn.1671-8925.2010.09.008
- VernacularTitle:PEP-1-SOD1预处理对脑梗死小鼠顶叶皮质的保护作用
- Author:
Min DONG
1
;
Gang-Ming XI
;
Wen-Chun WU
;
Ya-Jun CAO
;
Hong-Yun LI
Author Information
1. 湖北省襄樊市中医院
- Keywords:
PEP-l;
PEP-1-SOD1;
Cerebral infarction
- From:
Chinese Journal of Neuromedicine
2010;09(9):897-900
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuropretective effect of PEP-1-SOD1 pretreatment on the parietal cortex of mice with cerebral infarction. Methods Healthy Kunming-mice were assigned randomly into sham-operated group, model group and PEP-1-SOD1 precondition group (n=15). And the models of permanent middle cerebral artery occlusion (pMCAO) were established in the later 2 groups.The mice in the sham-operated group and model group were intraperitoneally injected with 200-ul saline and the mice in the PEP-1-SOD1 precondition group were intraperitoneally injected with 200-ug PEP-1-SOD1 fusion protein 30 min before the model inducement, respectively. The parietal cortex was dissected 24 h after the success of model making. Triphenyltetrazolium chloride (TTC) staining was employed to detect the volume of infarction and TUNEL staining was used to detect the cell apoptosis;the content of malondialdehyde (MDA) was measured using the thiobarbituric acid (TBA) method and the activity of SOD 1 was measured by xanthine oxidase method. Results The volume of infarction in the PEP-1-SOD1 precondition group was obviously smaller than that in the model group (P＜0.05).Compared with those in the model group, significantly reduced apoptotic neural cells were noted in the PEP-1-SOD1 precondition group (P＜0.05). Compared with model group, increased activity of SOD1 and decreased level of MDA were found in the cell apoptosis (P＜0.05). Conclusion Precondition with PEP-1-SOD1 fusion protein can efficiently protect the parietal cortex of mice with cerebral infarction.
