Association between tumor necrosis factor-α T1031C and C863A gene polymorphisms and ischemic stroke in elderly patients
10.3760/cma.j.issn.1671-8925.2010.09.007
- VernacularTitle:肿瘤坏死因子-α C863A、T1031C基因多态与缺血性脑卒中的相关性研究
- Author:
Hong-Wei YANG
1
;
Hong-Xing HAN
;
Yan-Chen XIE
;
Shu-Hui WANG
;
Yun ZHANG
;
Ming-Yi ZHANG
Author Information
1. 天津医科大学总医院
- Keywords:
Tumor necrosis factor-α;
Gene;
Polymorphism;
Ischemic stroke
- From:
Chinese Journal of Neuromedicine
2010;09(9):893-896
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the tumor necrosis factor-α(TNF-α) T1031C and C863A gene polymorphisms and senile ischemic stroke (IS) in elderly patients.Methods One hundred and fifty patients with IS and 110 age and gender-matched controls were recruited in this study. The T1031C and C863A gene polymorphisms were detected by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in these subjects. Data were coded and analyzed in SPSS Windows (version 11.5): the differences of genotype and allele frequencies were analyzed and compared byx2 test; multiple logistic regression analysis was employed to analyze the effect of various risk factors on IS. Results The frequencies of CA+AA genotypes (0.387) and A allele (0.203) at the position of-863 in the IS group were significantly higher than those in the control group ([0.255 and 0.127]; [χ2=5.004, P=0.025] and [χ2=5.176, P=0.023]). Further analysis indicated that the frequencies of genotypes and alleles were statistically different between male IS subgroup (0.430 and 0.227) and controls ([0.212 and 0.106]; [x=7.968, P=0.005] and [χ2=7.557, P=0.006]). No differences of TC+CC genotypes and C alleles at the position of-1031 were observed between IS group and controls ([χ2=1.463, P=).226] and [χ2=2.849, P=0.091]). Multiple logistic regression analysis revealed that hypertension, diabetes mellitus, hyperlipidemia and the A allele of C863A gene polymorphism were independent risk factors for IS in elderly patients (P=0.022, OR=1.846, 95%CI: 1.075～3.169).Conclusion The TNF-α C863A gene polymorphism may be an independent risk factor for senile IS.The T1031C gene is unlikely to contribute to the IS.
