Umbilical cord blood cell-derived neurospheres differentiating into Schwann-like cells
10.3760/cma.j.issn.1671-8925.2010.09.005
- VernacularTitle:脐血源性神经球分化为类雪旺细胞的研究
- Author:
Yong-Ming LI
1
;
Peng ZHANG
;
Hong-Tian ZHANG
Author Information
1. 河南省人民医院
- Keywords:
Umbilical cord blood;
Cell differentiation;
Schwann cell;
Co-culture
- From:
Chinese Journal of Neuromedicine
2010;09(9):883-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the phenotypic and biological characteristics of human umbilical cord blood mesenchymal stem cells (CB-MSCs) differentiating into a Schwann cell lineage.Methods Initially, we induced human CB-MSC into floating neurospheres,and then, neurospheres were induced to differentiating into Schwann-like cells using glia growth factors.Differentiated CB-MSCs showed morphological changes eing similar to those of Schwann cells.Expression of the Schwann cell markers was determined by immunocytochemical staining and Western blotting.Furthermore,differentiated CB-MSCs were co-cultured with dorsal root ganglia neurons to promote the neurite outgrowth.Results The stained nestin,GFAP and S-100 of the undifferentiated CB-MSCs were barely expressed. When CB-MSCs were induced into neurospheres,the nestin positive cells were (82.38±3.70)％,but the expressions of GFAP and S-100 were still negative.After inoculated 36 h,the positive rates of nestin, GFAP,S-100 were (43.78±3.21)％,(35.42±1.82)％ and (29.49±2.54)％,respectively.Compared to those of the undifferentiated group,differentiated axons of CB-MSC were significantly longer and the numbers of them were obviously larger (P＜0.05).Conclusion CB-MSCs could be onverted into neurospheres and then induced into Schwann-like cells in terms of morphology,phenotype and functional capacities.CB-MSCs can be used as an alternative source for bone marrow derived-MSCs,suggesting their potential values in the treatment of neurological diseases.
