Expression changes of peroxisome proliferator activated receptor γ following secondary injuries in rats with spinal cord injury
10.3760/cma.j.issn.1671-8925.2010.09.004
- VernacularTitle:过氧化物酶体增殖物激活受体γ在大鼠脊髓损伤后继发性损伤中的表达变化
- Author:
Fa-Chen WANG
1
;
Shao-Ji YUAN
;
Hua FENG
;
Rong HU
Author Information
1. 济南军区总医院
- Keywords:
Peroxisome proliferator activated receptor;
Spinal cord injury;
Secondary injury
- From:
Chinese Journal of Neuromedicine
2010;09(9):879-882
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression changes of peroxisome proliferator activated receptor γ(PPARγ) following the secondary injuries in rats with spinal cord injury (SCI). Methods Seventy-two male SD rats were equally randomized into control group and SCI group. Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was performed to detect the mRNA expression changes of PPARγ on the 1st, 3nd, 7th 14th, 28th and 56th d of SCI. Western blotting wasemployed to detect the protein expression changes of PPARγ Results Compared with those in the control group, the mRNA and protein expressions of PPARγ in the SCI group on the 1st, 3rd, 7th, 14th and 28th d of SCI were significantly increased (P＜0.05), reaching its peak level 14 d after SCI; on the 56th d of SCI, their expression levels in the SCI group were still higher than those in the control group, but no significant differences were noted (P＞0.05). Conclusion The expressions of PPARγare significantly increased following secondary injuries in rats with spinal cord injury, reaching its peak level 14 d after SCI.
