Expression and significance of microRNA-134 in mouse brain tissue with FMR1 gene knockout
10.3760/cma.j.issn.1671-8925.2010.08.003
- VernacularTitle:MicroRNA-134在FMR1基因敲除鼠脑组织中的表达及意义
- Author:
Zhi-Yong ZENG
1
;
Wei DI
;
Du XIAO
;
Xun-Sha SUN
;
Yu-Liang WANG
;
Mei OU-YANG
;
Yong-Hong YI
Author Information
1. 深圳市龙岗区布吉人民医院
- Keywords:
Fragile X syndrome;
MicroRNAs;
Fragile X mental retardation protein
- From:
Chinese Journal of Neuromedicine
2010;09(8):765-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression ofmicroRNA-134 (miR-134) in the mouse brain tissue with FMR1 gene knockout during the different development periods and its expression characteristic, and explore whether the deficiency of fragile X mental retardation protein (FMRP) can induce the changes of miR-134 transcription. Methods FVB strain male mice, including FMR1 gene knockout (KO, n=15) and their wild type (WT, n=15) counterparts were chosen in the experiment. The expressions of miR-134 in the brain tissues of these KO mice that were 0 d, 4 and 6 w old and the age-matched WT mice were detected by qRT-PCR. Results The transcriptional level of miR-134 in the brain tissue of KO mice had no significant difference as compared with that of age-matched WT mice (P＞0.05). The transcriptional levels of miR-134 in 6-w-old KO and WT mice were significantly decreased as compared with the newbom and 4-w-old same genotype mice (P＜0.05). Conclusion The absence of FMRP does not influence the transcription of miR-134 and the transcriptional level of miR-134 in the brain tissues maintains a high level during the developmental stage of the nervous system and gradually decreases to a low level after grow-up, demonstrating its important role in regulating the development of nervous system.
