Effects of mild hypothermia on heat shock protein 70, interlukin 6 and tumor necrosis factor-α of blood and cerebrospinal fluid in patients with cerebroma
10.3760/cma.j.issn.1671-8925.2010.07.019
- VernacularTitle:亚低温对脑肿瘤患者血液和脑脊液热休克蛋白70、IL-6和TNF-α的影响
- Author:
Jian RONG
1
;
Sheng YE
;
Nan JIANG
;
Jie-Yu FANG
;
Jun-Ying GUO
;
Liang-Can XIAO
Author Information
1. 中山大学附属第一医院
- Keywords:
Cerebral tumor;
Mild hypothermia;
Heat shock protein 70;
Iinterlukin 6;
Tumor necrosis factor-α
- From:
Chinese Journal of Neuromedicine
2010;09(7):723-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of mild hypothermia on the heat shock protein 70 (HSP70), interlukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) in blood and cerebrospinal fluid in patients with cerebroma and explore the molecular mechanism of hypothermia on cerebral ischemia. Methods Forty patients, selected for cerebroma resection under microscopy, were randomized innormothermia group (n=20, 36.6±0.4 ℃) and hypothermia group (n=20,34.8±0.6 ℃). These temperatures were guaranteed by air temperature control blanket. Jugular venous blood and cerebrospinal fluid were collected before the anesthesia induction (T1), 1 h after opening the endocranium (T2) and 24 h after the operation (T3). The content of HSP70, IL-6 and TNF-α in the blood and cerebrospinal fluid was measured by ELISA, and the results were statically analyzed. Results The core temperature in the hypothermia group was decreased significantly from starting the operation to the end of tumor resection as compared with that in the normothermia group (P<0.05). The level of HSP70 in blood or cerebrolspinal fluid presented an increasing trend in both groups, and the level of HSP70 at T2 and T3 in the hypothermia group was significantly higher than that in the normothermia group (P<0.05). Compared with those in the normothermia group, the levels of IL-6 and TNF-α in blood or cerebrospinal fluid at T2 and T3 in the hypothermia group were significantly lower (P<0.05). At T1 and T2 in the normothermia group, the content of HSP70 in cerebrospinal fluid was obviously higher than that in blood (P<0.05).Conclusion Mild hypothermia can increase the expression level of HSP70 in blood and cerebrospinal fluid and decrease the level of IL-6 and TNF-α in blood and cerebrospinal fluid. HSP70 in cerebrospinal fluid might be more sensitive than that in blood in presenting the brain injury.
