Effect of transplantation of umbilical cord blood mesenchymal stem cells on traumatic brain injury in rats
10.3760/cma.j.issn.1671-8925.2010.07.008
- VernacularTitle:脐带血间充质干细胞移植对大鼠脑创伤的影响
- Author:
Peng ZHANG
1
;
Lai-Jun SONG
;
Bo YANG
Author Information
1. 郑州大学附属第一医院
- Keywords:
Mesenchymal stem cells transplantation;
Craniocerebral trauma;
Cell differentiation;
Neuron-like cells
- From:
Chinese Journal of Neuromedicine
2010;09(7):678-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of human umbilical cord blood mesenchymal stem cells (CB-MSCs) in differentiating into neural-like cells and the effect of CB-MSC transplantation on traumatic brain injury in rats. Methods Healthy Wistar rats were induced into model with experimental traumatic brain injury by drilling and hitting their brain tissue, and then, they were randomized into 3 groups (n=18): model group, control group (injured models + injecting 1.25 μL saline) and CB-MSC transplantation group (injured model + injecting CB-MSC suspension). CB-MSC were derived from separated umbilical cord blood, cultured, marked with BrdU and injected into injured area of rats in the CB-MSC transplantation group. The motor function scale was performed 3 and 10 d after the transplantation, and Y maze test was employed to observe the rat's learning and memory abilities 2 and 4 w after the transplantation. CB-MSC in the CB-MSC transplantation group was detected by immunohistochemistry 2 and 5 w after the transplantation; BrdU-GFAP and BrdU-NSE positive cells were observed. Results Significant differences between the 3 groups were observed in motor function scores on the 10th day of transplantation and in rat's learning and memory abilities with Y maze test 2 and 4 w after the transplantation. BrdU-GFAP and BrdU-NSE positive cells were found in the area of transplantation in the CB-MSC transplantation group only by the end of 2 and 5 w after transplantation. Conclusion CB-MSC transplantation can help the recovery of brain injury in rats and improve the learning and memory abilities; CB-MSC transplanted into the rats' brain tissue can differentiate into neurons-like cell in vivo.
