Regulation of Rac1 expression affecting cell proliferation and apoptosis of Daoy cells in medulloblastoma
10.3760/cma.j.issn.1671-8925.2010.07.007
- VernacularTitle:Rac1表达调控对髓母细胞瘤Daoy细胞增殖与凋亡的影响
- Author:
Bao-Dong CHEN
1
;
Yong-Zhong GAO
;
Tai-Peng JIANG
;
Jian-Jun DING
;
Xiao-Dan JIANG
;
Ru-Xiang XU
Author Information
1. 广东省深圳市第二人民医院
- Keywords:
Rac1 gene;
RNA interference;
Cell cycle;
Apoptosis;
Medulloblastoma
- From:
Chinese Journal of Neuromedicine
2010;09(7):674-677
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Rac1 gene silencing on cell cycle and apoptosis of Daoy cell line in medulloblastoma. Methods Daoy cells were divided into Rac1-shRNA group and empty plasmid control group; Daoy cells in the former group were transfected by using Rac1-shRNA plasmid (Rac1-shRNA). RT-PCR, Western blotting and flow cytometry were used to observe the changes of cycle and apoptosis of Daoy cells after Rac1 gene silencing. Results Rac1 mRNA and protein in medulloblastoma Daoy cell lines were highly expressed; cell cycle of Daoy cells with Rac1 gene silencing were blocked at G0-G1 phases and cell percentage of G0-G1 phases significantly increased to 80.9%±4.9%; however, the proportion of cells in the S phase reduced to 11.8%± 2.3%. The apoptosis rate of Rac1-shRNA plasmid group (36.7%±3.9%) was significantly different as compared with that of empty plasmid control group (8.5% ±0.9%) (P<0.05). Conclusion RNA-interfered Rac1 gene silencing can inhibit the proliferation of Daoy cells and promote their apoptosis, indicating that Rac1 may become a new target being able to inhibit the cell proliferation and promote the apoptosis of Daoy cells in medulloblastoma.
