Preliminary greening of specificity-associated genes of pituitary adenoma subtypes by whole genome oligonucleotide microarray
10.3760/cma.j.issn.1671-8925.2010.06.009
- VernacularTitle:利用全基因组芯片初步筛选亚类垂体腺瘤特异性相关基因
- Author:
Xiao-Qiang GAN
1
;
Xue-Hua DING
;
Yi-Cheng LU
;
Guo-Han HU
;
Ju-Xiang CHEN
;
Hua-Sheng XIAO
Author Information
1. 第二军医大学附属长征医院
- Keywords:
Pituitary adenornas;
Oligonucleotide microarray;
Gene expression profile
- From:
Chinese Journal of Neuromedicine
2010;9(6):576-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression and function of correlative genes in the happening and developing of human pituitary adenoma-subtypes.Methods The whole genome oligonucleotide microarray (Affymetrix 133 plus 2.0) was used to examine the gene expressions of pituitary adenoma tissue in 8 patients with pituitary adenoma (2 with growth hormone adenomas,2 with prolactinomas,2 with gonadotroph adenomas and 2 with null cell adenomas) and normal pooled pituitary tissue.Differentially expressed genes were analyzed by Hierarchical method and bioinformatics.A candidate gene was selected to verify the microarray analyzed result by real-time quantitative PCR.Results Compared with associated genes with normal control,associated genes with pituitary adenoma mainly involved in the following biological processes analyzed from the view of function: binding,apoptosis-or-tumor correlation,metabolism,signal-transducer-activity,cell cycle,transcription-regulator-activity and transporter-activity.The specificity of expression in several differential genes was connected to the development of pituitary adenoma-subtypes.Conclusion The development of pituitary adenoma is a complex regulation process involving lots of genes,molecules and pathways.However,the molecular mechanism related to the individual pituitary adenoma-subtypes is different.
