Immunogenicity of recombinant DNA vaccine that coded for the neurite growth inhibitors
10.3760/cma.j.issn.1671-8925.2010.05.001
- VernacularTitle:编码轴突生长抑制因子的重组DNA疫苗的免疫原性研究
- Author:
Sheng-Bin KOU
1
;
Xiao-Dan JIANG
;
Yan-Ping TANG
;
Ying-Qian CAI
;
Mou-Xuan DU
;
Ling-Sha QIN
;
Yu-Xi ZOU
;
Ru-Xiang XU
Author Information
1. 南方医科大学珠江医院
- Keywords:
Neurite growth inhibitor;
DNA vaccine;
Immunogenicity;
Axonal regeneration
- From:
Chinese Journal of Neuromedicine
2010;9(5):433-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the immunogenicity of the recombinant DNA vaccine that encoded for neurite growth inhibitors: Nogo-A, oligodendrocyte myelin glycoprotein (OMgp), tenascin-R (TN-R) and myelin-associated glycoprotein (MAG) after the nerve injury under the help of pAdEasy, a kind of adenovirus plasmid being the vector of the DNA. Methods Sixteen 5-w-old Lewis rats were randomized into DNA vaccination group (vaccine group) and pAdEasy group. Rats in the vaccine group were immunized once weekly for a consecutive 8 w by bilateral injection of the recombinant plasmid into the musculus tibialis. The immunized animals in the 2 groups were exsanguinated each time before the vaccination for sera collection, and the qualitation and quantitation of the antibodies in the serum were detected by Dot-blot analysis and ELISA. Results The vaccine group could produce fusion-protein antibodies against Nogo-A, MAG, OMgp and TN-R at the 6th w of vaccine injection, while pAdEasy group could not. The valency of antiserum was shown by ELISA as 1:1 000 000 at the 6th w of vaccine injection and kept this level stably. Conclusion The DNA vaccine exclusively induces the generation of the fusion-protein antibodies against Nogo-A, MAG, OMgp and TN-R in vivo, which controls the favorable immunogenicity.
